missing translation for 'onlineSavingsMsg'
Learn More
Gibco™ Human Dkk-1 Recombinant Protein Product Code.: 10023534
  1. Home
  2. Products
  3. Recombinant Proteins
  4. Gibco™ Human Dkk-1 Recombinant Protein

Gibco™ Human Dkk-1 Recombinant Protein

Product Code. 10023534
10 μg, Each
Click to view available options
Quantity:
1 mg
10 μg
100 μg
25 μg
Unit Size:
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10023534

Brand: Gibco™ PHC9214

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water to a concentration of 0.1-0.5 mg/mL. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store the lyophilized protein at 2-8°C or -20°C long term, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O94907
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation protein with no preservative
Gene ID (Entrez) 22943
Molecular Weight (g/mol) 42 kDa
Name Human Dkk-1
Purification Method Sequential Chromatography
Quantity 10 μg
Endotoxin Concentration <0.1 ng/ μg
Gene Alias dickkopf 1 homolog; dickkopf homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; dickkopf WNT signaling pathway inhibitor 1; Dickkopf1; dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; dickkopf-related protein 1; DKK1; DKK-1; hDkk1; hDkk-1; mdkk-1; SK; UNQ492/PRO1008
Common Name DKK1
Biological Activity ED50 ≤ 40 ng/mL; determined by the binding of immobilized human LRP6/Fc chimera receptro (3 μg/mL) to human Dkk-1 in. a functional ELISA.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Content And Storage 4°C
Classification Carrier-Free
Endotoxin Level <0.1 ng/μg
Reactivity Human
Shipping Condition Wet Ice
Specific Activity Linear range of 0.3 to 40 ng⁄ml
Expression System HEK293 cells
Protein Family Cytokines & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype Other Proteins
Research Category Oncology, Signal Transduction
Product Line Gibco™
Purity or Quality Grade ≥95% by SDS-PAGE and HPLC
Show More Show Less

Product Suggestions

Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

4 results found
Lot Number Certificate Type Date Product Code
Lot Number2797313 Certificate TypeCertificate of Analysis Date01/03/2024 Product CodePHC9214
Lot Number2529563 Certificate TypeCertificate of Analysis Date23/07/2022 Product CodePHC9214
Lot Number2565374 Certificate TypeCertificate of Analysis Date23/07/2022 Product CodePHC9214
Lot Number1309130A Certificate TypeCertificate of Analysis Date13/04/2017 Product CodePHC9214
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Gibco™ Human Dkk-1 Recombinant Protein >

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.