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Cytiva HiScreen™ Capto™ Phenyl (High Sub) Prepacked HIC Column
Hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification, used for method optimization and parameter screening
Brand: Cytiva 28-9924-72
This item is not returnable.
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Description
- Highly cross-linked agarose matrix with average bead size of 75μm
- Has good flow properties and high loading capacities
- Bed height of 10cm designed to allow method optimization and parameter screening
- Connect easily in series to achieve 20cm bed height
- Small bed volume gives fast results and minimal sample/buffer consumption
- Capto Phenyl (high sub) media is used for initial and intermediate step purifications requiring a chromatography medium with medium to high hydrophobicity
- Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
- Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity
- Bed volume: 4.7mL
- Bed dimension: 7.7 × 100mm
- Flow rate: 1.2 to 2.7mL/min.
- Flow velocity: <600cm/hr.
- Average particle size: 75μm
- Degree of substitution: 27μmol/mL gel
- pH stability cleaning: 2 to 14
- Pressure/Flow specification: 300kPa at 600cm/hr., 1m diameter column, 20cm bed height
- Medium: BioProcess
- pH stability working range: 3 to 13
- Ligand: phenyl
- Binding capacity/mL chromatography medium: 27mg BSA /mL (at QB10%)
- Bed support: filter
- Material (column hardware and column tube): polypropylene
- Column I.D.: 7.7mm
Specifications
| Aqueous Buffer | |
| 4.7mL | |
| 7.7mm | |
| 3.93 in. | |
| <600cm/hr. | |
| Phenyl (∼27μM/mL drained medium) | |
| 3 to 13 | |
| Highly Cross-linked Agarose | |
| HIC Column |
| HiScreen Capto Phenyl (high sub) column | |
| 0.3 in. | |
| 1 x 4.7mL | |
| 100mm | |
| Polypropylene | |
| 43.5psi | |
| 75μm | |
| 4° to 30°C | |
| Optimization and parameter screening |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HiScreen Capto Phenyl (high sub), 1 x 4.7 ml
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol