missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ Human IL-29 (IFN lambda 1) Uncoated ELISA Kit
ELISA
Brand: Invitrogen™ 88-7296-77
This item is not returnable.
View return policy
Description
The Human Interleukin 29 (IFN lambda 1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-29. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
IFN-lambdas constitute a novel family of interferons that mediate the induction of anti-viral protection in a wide variety of cells. There are three members in this family, namely lambda1, lambda2, and lambda3, which are also known as IL-29, IL-28A, and IL-28B, respectively. Similar to type I IFNs, IFN-lambdas activate an intracellular signaling to promote gene expression. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex composed of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. DCs that differentiate in response to IFN-lambdas express high levels of class I and II MHC gene products, but low levels of costimulatory molecules. Moreover, these cells can specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cells with contact-dependent suppressive activity.
Specifications
| Q8IU54 | |
| 8 pg/mL | |
| ELISA | |
| Human | |
| Colorimetric Microplate Reader | |
| BSF-1,IL-4 | |
| HRP | |
| RUO | |
| 2°C to 8°C | |
| IL-29 | |
| 24 hrs 30 mins |
| 8 to 1,000 pg/mL | |
| HRP | |
| Plasma, Serum, Supernatant | |
| ELISA | |
| 282618 | |
| Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 ÎĽM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) | |
| 20 x 96 Tests | |
| Plasma 100 ÎĽL, Serum 100 ÎĽL, Supernatant 100 ÎĽL | |
| Interleukin-29 | |
| 1 hrs 45 mins |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Invitrogen™ Human IL-29 (IFN lambda 1) Uncoated ELISA Kit > 20 x 96 Tests; HRP
Spot an opportunity for improvement?Share a Content Correction