IgM, eFluor 570, Clone: II/41, eBioscience™

Description

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: This II/41 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunocytochemistry.Applications Tested: This II/41 antibody has been tested by immunocytochemistry on fixed, differentiated OLN93 cells with O1 (Anti-Oligodendrocyte Marker) primary antibody, and by immunohistochemistry (IHC-P) on FFPE human tonsil with HI98 (Anti-CD15) as the primary antibody. This II/41 antibody can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm