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Invitrogen™ PureLink™ HiPure Plasmid Filter Maxiprep Kit

The PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of overnight bacterial culture.

3490.00 SEK - 8030.00 SEK

Specifications

Column Type Filter Column
Content And Storage The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.
Format Column
Isolation Technology Anion Exchange Resin
Sample Type Bacterial Culture
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Includes: The PureLink HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns.

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10043432
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Invitrogen™
K210016
10 Preps
3490.00 SEK
120mL
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Invitrogen™
K210017
25 Preps
8030.00 SEK
Each
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Description

Description

The PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of overnight bacterial culture. The PureLink HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of bacterial lysate with anion-exchange resin purification of plasmid DNA, in single unit. The resulting plasmid DNA is of quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification.

  • Purify high-quality DNA in Less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins
  • Additionally, elimination of phenol, chloroform, ethidium bromide, and cesium chloride from protocol minimizes exposure to and disposal of hazardous materials

PureLink HiPure Plasmid Filter Kits provide:

  • Simple protocols—bacterial lysate clarification without centrifugation
  • Fast results save time by purifying plasmid DNA in less than two hours
  • High yields up to 200 μg for midipreps and up to 850 μg for maxipreps
  • Reliable performance, high-quality plasmid DNA purified with these kits is suitable for wide variety of applications

How it works

  • Addition of a HiPure Filter Column to the PureLink HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation
  • Lysate filtration cartridge is integrated into DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin
  • Single column wash removes impurities such as RNA, proteins, metabolites, and other low molecular weight molecules; the ultrapure plasmid DNA is then eluted with high-salt buffer
  • DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Filter Column
Column
Bacterial Culture
15 mL
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
<40kb, Low Copy Plasmid, High Copy Plasmid, BAC
PureLink™
Transfection Grade
Plasmid DNA
90 min.
The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.
Anion Exchange Resin
Maxi
Plasmid DNA
Not High-throughput Compatible (Manual)
> 200 μg (Large-Scale) Plasmid DNA
Plasmid Filter MaxiPrep Kit
Room Temperature
PureLink™
1000 μg
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.