DescriptionRestore PLUS Buffer is an alternative formulation of the original Thermo Scientific Restore Western Blot Stripping Buffer. Restore PLUS Stripping Buffer was designed for use with antibodies that are difficult to remove from Western blots and require longer incubation times or incubation temperatures greater than 22°C with gentler formulations. High-affinity antibodies can be quickly and effectively stripped from Western blots at room temperature without removing transferred proteins, thereby allowing multiple reprobes of the target.
Ready and easy to use – no dilution necessary; no offensive odors; store at room temperature
Compatible – use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate
Cost effective – save valuable time and samples; strip blots effectively the first time
Robust yet gentle – transferred proteins remain viable; strip the same blot up to five times
Flexible – strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies