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IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

2280.00 SEK - 3150.00 SEK

Specifications

Format Liquid
For Use With (Equipment) StarGate™ System, Yeast Host Cells
Inducing Agent Copper sulfate
Marker Gene leu2-d and URA3
pH 8
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Products 21
Product Code Brand Description Protein Tag Price Quantity & Availability  
Product Code Brand Description Protein Tag Price Quantity & Availability  
15613808
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IBA Lifesciences
5-4600-001
pYSG-IBA wt1 Untagged
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15623808
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IBA Lifesciences
5-4603-001
pYSG-IBA 3 Strep-tag for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15633808
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IBA Lifesciences
5-4605-001
pYSG-IBA 5 Strep-tag for N-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15643808
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IBA Lifesciences
5-4623-001
pYSG-IBA 23 Strep-tag for C-terminal and GST-tag for N-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15673808
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IBA Lifesciences
5-4643-001
pYSG-IBA 43 His Tag (6x) for N-terminal and Strep-tag for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15683808
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IBA Lifesciences
5-4645-001
pYSG-IBA 45 Strep-tag for N-terminal and His Tag (6x) for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15693808
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IBA Lifesciences
5-4662-001
pYSG-IBA 62 Strep-tag for C-terminal and FLAG-tag for N-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15613818
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IBA Lifesciences
5-4664-001
pYSG-IBA 64 Strep-tag for N-terminal and FLAG-tag for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15633818
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IBA Lifesciences
5-4703-001
pYSG-IBA 103 Twin-Strep-tag for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15643818
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IBA Lifesciences
5-4705-001
pYSG-IBA 105 Twin-Strep-tag for N-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15653818
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IBA Lifesciences
5-4723-001
pYSG-IBA 123 Twin-Strep-tag for C-terminal and GST-tag for N-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15663818
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IBA Lifesciences
5-4743-001
pYSG-IBA 143 His Tag (6x) for N-terminal and Twin-Strep-tag for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15673818
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IBA Lifesciences
5-4745-001
pYSG-IBA 145 Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15683818
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IBA Lifesciences
5-4762-001
pYSG-IBA 162 Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15693818
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IBA Lifesciences
5-4764-001
pYSG-IBA 164 Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15603828
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IBA Lifesciences
5-4767-001
pYSG-IBA 167 Twin-Strep-tag/FLAG-tag for N-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15613828
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IBA Lifesciences
5-4768-001
pYSG-IBA 168 Twin-Strep-tag/FLAG-tag for C-terminal
3150.00 SEK
5µg
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Estimated Shipment: 15-04-2024
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15603818
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IBA Lifesciences
5-4663-001
pYSG-IBA 63 FLAG-tag for C-terminal This item is currently unavailable or has been discontinued.
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15663808
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IBA Lifesciences
5-4633-001
pYSG-IBA 33 His Tag (6x) for C-terminal This item is currently unavailable or has been discontinued.
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15623818
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IBA Lifesciences
5-4665-001
pYSG-IBA 65 FLAG-tag for N-terminal This item is currently unavailable or has been discontinued.
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N/A View alternative products
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15653808
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IBA Lifesciences
5-4625-001
pYSG-IBA 25 GST Tag for N-terminal This item is currently unavailable or has been discontinued.
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Description

Description

pYSG-IBA yeast expression vectors

The pYSG-IBA vector allows the high-level expression of proteins in yeast.

  • The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
  • The expressed recombinant protein will be localized in the cytoplasm.

 

  • High-level expression in yeast with high copy number
  • Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
  • Tightly regulated expression due to Cu++-inducible CUP1 promoter
  • Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
  • Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
  • URA3 auxotrophy marker for selection after transformation

Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Specifications

Liquid
Copper sulfate
8
Acceptor Vector for yeast expression
2°C to 8°C for frequent usage, -20°C for long-term storage
5 μg
Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
StarGate™ System, Yeast Host Cells
leu2-d and URA3
CUP1
20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
250 ng/μL
Clear
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Documents

Product Certifications