missing translation for 'onlineSavingsMsg'
Learn More

IBA Solutions For Life Science StarGate™ pYSG-IBA Acceptor Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

2024.00 SEK

Specifications

Promoter CUP1
Inducing Agent Copper sulfate
Quantity 5 μg
Concentration 250 ng/μL
Storage Buffer 20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μg
View More Specs

Products 21
Product Code Brand Description Protein Tag Price Quantity & Availability  
Product Code Brand Description Protein Tag Price Quantity & Availability  
15613808 View Documents IBA Solutions For Life Science
5-4600-001
pYSG-IBA wt1 No affinity tag 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15623808 View Documents IBA Solutions For Life Science
5-4603-001
pYSG-IBA 3 Strep-tag™ for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15633808 View Documents IBA Solutions For Life Science
5-4605-001
pYSG-IBA 5 Strep-tag™ for N-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15643808 View Documents IBA Solutions For Life Science
5-4623-001
pYSG-IBA 23 GST-tag for N-terminal and Strep-tag™ for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15653808 View Documents IBA Solutions For Life Science
5-4625-001
pYSG-IBA 25 GST-tag for N-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15663808 View Documents IBA Solutions For Life Science
5-4633-001
pYSG-IBA 33 6xHistidine-tag for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15673808 View Documents IBA Solutions For Life Science
5-4643-001
pYSG-IBA 43 6xHistidine-tag for N-terminal and Strep-tag™ for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15683808 View Documents IBA Solutions For Life Science
5-4645-001
pYSG-IBA 45 Strep-tag™ for N-terminal and 6xHistidine-tag for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15693808 View Documents IBA Solutions For Life Science
5-4662-001
pYSG-IBA 62 FLAG-tag for N-terminal and the Strep-tag™ for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15603818 View Documents IBA Solutions For Life Science
5-4663-001
pYSG-IBA 63 FLAG-tag for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15613818 View Documents IBA Solutions For Life Science
5-4664-001
pYSG-IBA 64 Strep-tag™ for N-terminal and FLAG-tag for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15623818 View Documents IBA Solutions For Life Science
5-4665-001
pYSG-IBA 65 FLAG-tag for N-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15633818 View Documents IBA Solutions For Life Science
5-4703-001
pYSG-IBA 103 Twin-Strep-tag™ for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15643818 View Documents IBA Solutions For Life Science
5-4705-001
pYSG-IBA 105 Twin-Strep-tag™ for N-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15653818 View Documents IBA Solutions For Life Science
5-4723-001
pYSG-IBA 123 GST-tag for N-terminal and Twin-Strep-tag™ for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15663818 View Documents IBA Solutions For Life Science
5-4743-001
pYSG-IBA 143 6xHistidine-tag for N-terminal and Twin-Strep-tag™ for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15673818 View Documents IBA Solutions For Life Science
5-4745-001
pYSG-IBA 145 Twin-Strep-tag™ for N-terminal and 6xHistidine-tag for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15683818 View Documents IBA Solutions For Life Science
5-4762-001
pYSG-IBA 162 FLAG-tag for N-terminal and Twin-Strep-tag™ for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15693818 View Documents IBA Solutions For Life Science
5-4764-001
pYSG-IBA 164 Twin-Strep-tag™ for N-terminal and FLAG-tag for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15603828 View Documents IBA Solutions For Life Science
5-4767-001
pYSG-IBA 167 FLAG-Twin-Strep-tag™ for N-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
15613828 View Documents IBA Solutions For Life Science
5-4768-001
pYSG-IBA 168 Twin-Strep-tag™-FLAG-tag for C-terminal 2024.00 SEK
5µg View alternative products
Save Up to 
»
Estimated Shipment
03-10-2019
Add to basket
 
Description

Description

pYSG-IGA yeast expression vectors

The pYSG-IBA3 vector allows the high-level expression of proteins in yeast. The vector carries the copper inducible promoter (CUP1) for controlled high-level expression. The expressed recombinant protein will be localized in the cytoplasm.

  • High-level expression in yeast with high copy number
  • Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
  • Tightly regulated expression due to Cu++-inducible CUP1 promoter
  • Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
  • Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
  • URA3 auxotrophy marker for selection after transformation

StarGate™ System

IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:

  • Minimal extra modification of the gene due to short combinatorial sites
  • Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
  • GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
  • Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
  • The final expression vector, the Destination Vector, is then placed into the respective host
Specifications

Specifications

CUP1
5 μg
20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μg
Liquid
Clear
StarGate™ system, yeast host cells
2-8°C for frequent usage, -20°C for long-term storage
Copper sulfate
250 ng/μL
8
2μ ori
Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins
Acceptor Vector for yeast expression