Invitrogen™ BioPrime™ Total Genomic Labeling System

Description

These complete, all-inclusive kits are optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component limited reaction allow for consistent, robust DNA yields of ∽8μg with as little as 50ng of genomic DNA input. An optimized dye labeled nucleotide mix with new dNTP linkers, novel, application-specific Alexa Fluor 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. Complete kits are batch tested to assure quality in performance from lot to lot.
Contents and Storage:
The simplified kits contains Alexa Fluor 3 and Alexa Fluor 5 2x reaction mix, Exo-Klenow fragment (40U/μL), 5mM EDTA, TE Buffer, and control DNA (Salmon Sperm). The BioPrime Total Genomic Labeling System also contains Purelink^{TM Genomic DNA purification columns and purification buffers (the BioPrime Total Genomic Labeling Module does not). New master mix formulation streamlines reaction set-ups, increasing consistency.}

• Higher signal to noise with Alexa Fluor™ 3 and 5 dyes
• Less channel bias due to novel reaction formulation
• Widest range of input material (50ng - 3μg)
• Simplified workflow with master mix formulation

Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research