Protein Extraction and Purification

GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors THROMBIN PROTEASE

Invitrogen™ Molecular Probes™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays

Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution PROTEIN QUANTITATION KIT ULTRASENSITIVE FLUORESCENce-based protein quantita PROBES KOMBO sufficient

GE Healthcare Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium. STREPTAVIDIN SEPHAROSE HP,5 ML

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors. HALT PROTEASE&PHOSPHAT INHIB 1MLmL

Thermo Scientific™ Pierce™ IP Lysis Buffer

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent. 250 ML PIERCE IP LYSIS BUFFER FOR 25,000CM2 MONOLAyer or 25g cell paste 250mL Store in fridge at 4C

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories MAGNETIC STAND-96 1 STANDFor the paramagnetic bead precipitation from

GE Healthcare Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. GE Healthcare Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards. GEL FILTRATION CAL KIT HMW

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Alfa Aesar™ Lysis Buffer, 0.5% Tween 20, pH 8.0

For isolation of His-tag proteins 500ML Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins 500ml

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. HALT PROTEASE INHIB 1-USE 2400µL(24x 100µL)

Thermo Scientific™ Pierce™ PES Protein Concentrators

Concentrate and desalt biological samples and perform buffer exchange with disposable polyethersulfone (PES)-membrane ultrafiltration centrifugal devices. X25 Protein concentrator PES 30K MWCO 0.5mL (pack

GE Healthcare C Columns

C columns are economical modular columns for low pressure or gravity feed, providing even distribution of eluent to the medium bed, even without flow adapters. COLUMN C 10/40

GE Healthcare His SpinTrap™ Spin Columns and Kit

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. GE Healthcare Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns. X50 HIS SpinTrap, 100UL

GE Healthcare Mono Q PC, Columns Prepacked with MonoBeads™

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles. MONO Q PC 1.6/5

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques. X50 Funnel Pierce disposablepolypropylene (pack

Thermo Scientific™ Pierce™ Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets and Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.

X20 Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing. X2 Detergent removal spin plate, 96 well, for

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation. PIERCE STREPTAVIDIN MAGNETIC BEADS

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes. X200 CENTRIFUGE COLUMNS, 0,8 ML

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours. SUBCELLULAR PROTEIN FRACTIONATION

Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads

Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators. 1mL Anti-HA magnetic beads

GE Healthcare His Mag Sepharose™ Ni Magnetic Beads

Designed for efficient, small-scale purification/screening of histidine-tagged proteins from different sources GE Healthcare Lifescience™ His Mag Sepharose™ Ni Magnetic Beads enable high-capacity small-scale purification. X2 His Mag Sepharose Ni, 5x 1ml

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Holds up to 24 microcentrifuge tubes MAGNARACK 24each

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay. Pierce Chromogenic Endotoxin Quant Ki, 60 reactions

GE Healthcare Streptavidin HP MultiTrap™ Plates

Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates X4 STREPTAVIDIN HP MULTITRAP

GE Healthcare Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification. 5 ML NI SEPHAROSE(TM) 6 FAST FLOW 5ML STORE ATambient Store at Room Temperature

GE Healthcare Mono S PC, Columns Prepacked with MonoBeads™

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles. MONO S 4.6/100 PE

GE Healthcare Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. GE Healthcare Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step. SEPHADEX G-25 COARSE 500 GRS

GE Healthcare illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules.  GE Healthcare illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration. SEPHADEX G-100 DNA GR.100 G

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