Protein Extraction and Purification
Cytiva (Formerly GE Healthcare Life Sciences) Thrombin Protease
For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
Thermo Scientific™ RIPA Lysis and Extraction Buffer
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
Invitrogen™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays
Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution
Thermo Scientific™ Low Protein Binding Collection Tubes (1.5 mL)
Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL).
Cytiva (Formerly GE Healthcare Life Sciences) Streptavidin Sepharose™ High Performance Medium
Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.
Thermo Scientific™ Pierce™ Disposable Column Trial Pack
Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.
Cytiva (Formerly GE Healthcare Life Sciences) Streptavidin HP MultiTrap™ Plates
Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates
Cytiva (Formerly GE Healthcare Life Sciences) C Columns
C columns are economical modular columns for low pressure or gravity feed, providing even distribution of eluent to the medium bed, even without flow adapters.
Cytiva (Formerly GE Healthcare Life Sciences) His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads
Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.
Cytiva (Formerly GE Healthcare Life Sciences) His SpinTrap™ Spin Columns and Kit
Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns.
Cytiva (Formerly GE Healthcare Life Sciences) Mono Q PC, Columns Prepacked with MonoBeads™
Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. Cytiva Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles.
Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Merck Millipore Calbiochem™ Protease Inhibitor Cocktail Set III, EDTA-Free
Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
Cytiva (Formerly GE Healthcare Life Sciences) Mammalian Protein Extraction Buffer
After preparation may be used for most enzyme assays
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
Cytiva (Formerly GE Healthcare Life Sciences) Gel Filtration Calibration Kits
Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.
Cytiva (Formerly GE Healthcare Life Sciences) HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns
Designed for method optimization and parameter screening
Thermo Scientific™ Pierce™ Spin Columns - Screw Cap
Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).
Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads
Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups.
MP Biomedicals™ FastPROTEIN™ BlueMatrix
Easy lysis of bacterial cells to release recombinant proteins
Cytiva (Formerly GE Healthcare Life Sciences) Sephadex™ G-25 Media
Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.
Invitrogen™ Magnetic Stand-96
Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories
Cytiva (Formerly GE Healthcare Life Sciences) Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic
Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
Thermo Scientific™ PMSF Protease Inhibitor
Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.
Cytiva (Formerly GE Healthcare Life Sciences) Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column
Superdex 30 prep grade (PG) size exclusion chromatography (gel filtration) media prepacked into a HiLoad 26/600 column is optimized for the separation of proteins and peptides, but can also be used with success to separate oligosaccharides.