Protein Extraction and Purification

GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors THROMBIN PROTEASE

GE Healthcare Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium. STREPTAVIDIN SEPHAROSE HP,5 ML

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes. X200 CENTRIFUGE COLUMNS, 0,8 ML

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. HALT PROTEASE INHIB 1-USE 2400µL(24x 100µL)

GE Healthcare Mono Q PC, Columns Prepacked with MonoBeads™

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles. MONO Q PC 1.6/5

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 5KG Sodium Dodecyl Sulfate (SDS), Electrophoresis(White Powder), (as Fatty Alcohol Sulfate):

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets broad-spectrum protease and pghospahatase inhibitor formulation provides complete protection from dephosphorylation and proteolytic degradation. X20 Pierce Protease and Phoshpatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay. 30RXN Chromogenic Endotoxin Quant Kit

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques. X50 Funnel Pierce disposablepolypropylene (pack

GE Healthcare Mammalian Protein Extraction Buffer

500 ML MAMMALIAN PROTEIN EXTRACTION BUFFER 500MLstore at 4°C

Alfa Aesar™ Lysis Buffer, 0.5% Tween 20, pH 8.0

For isolation of His-tag proteins 500ML Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins 500ml

Thermo Scientific™ Pierce™ Lysozyme

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall. 0.5ML Lysozyme 50mg/mL

Thermo Scientific™ M-PER™ Mammalian Protein Extraction Reagent

Isolate total protein from cultured mammalian cells with this highly efficient lysis and extraction reagent. 1 LT MAMMALIAN PROTEIN EXTRACTION REAGENT M-PER1000mL

GE Healthcare His Mag Sepharose™ Ni Magnetic Beads

Designed for efficient, small-scale purification/screening of histidine-tagged proteins from different sources GE Healthcare Lifescience™ His Mag Sepharose™ Ni Magnetic Beads enable high-capacity small-scale purification. X5 His Mag Sepharose Ni, particle size 37µm to

CD63, clone: Ts63, Invitrogen™

Mouse Monoclonal Antibody 200UL EXOSOME - ANTI-CD63 WESTERN

GE Healthcare Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. GE Healthcare Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards. GEL FILTRATION CAL KIT LMW

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Holds up to 24 microcentrifuge tubes MAGNARACK 24each

GE Healthcare illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules.  GE Healthcare illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration. SEPHADEX G-100 DNA GR.100 G

GE Healthcare His SpinTrap™ Spin Columns and Kit

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. GE Healthcare Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns. X50 HIS SPINTRAP

GE Healthcare GST Bulk Kit

For small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins BULK GST PURIFICATION MODULE

GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). X5 HISTrap High performance 5mL

HEPES Lysis Buffer with Triton™ X-100, 2X

125ML HEPES lysis buffer with Triton|r X-100 (2X) 125ml

GE Healthcare MiniBeads™ Columns

MiniBeads™ prepacked columns are designed for high performance purification and characterization of biomolecules using ion exchange chromatography MINI Q PC 3.2/3

GE Healthcare Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification. 100ML Ni Sepharose 6 Fast Flow

GE Healthcare Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. GE Healthcare Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step. SEPHADEX G-25 SUPERFINE 100 GRS

GE Healthcare C Columns

C columns are economical modular columns for low pressure or gravity feed, providing even distribution of eluent to the medium bed, even without flow adapters. COLUMN C 16/100

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Molecular Probes™ CaptAvidin™ Agarose (Sedimented Bead Suspension)

CaptAvidin™ Agarose (Sedimented Bead Suspension) AGAROSE S CAPTAVIDIN(TM) STORE AT 2° TO 8°C

GE Healthcare HiTrap™ Capto™ Core 700 Columns

HiTrap Capto Core 700 is prepacked with Capto Core 700, a multimodal BioProcess medium for intermediate purification and polishing of viruses and other large biomolecules. X5 COLUMN HITRAP(TM) CAPTO(TM) CORE 700 1ML

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