Protein Extraction and Purification

Cytiva Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Invitrogen™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays

Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution

Thermo Scientific™ Low Protein Binding Collection Tubes (1.5 mL)

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL).

Cytiva Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.

Thermo Scientific™ Pierce™ Disposable Column Trial Pack

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

Cytiva Streptavidin HP MultiTrap™ Plates

Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates

Cytiva C Columns

C columns are economical modular columns for low pressure or gravity feed, providing even distribution of eluent to the medium bed, even without flow adapters.

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Merck Millipore PureProteome™ Magnetic Stands

Rapidly and easily isolate magnetic particles

Cytiva His SpinTrap™ Spin Columns and Kit

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns.

Cytiva Mono Q PC, Columns Prepacked with MonoBeads™

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. Cytiva Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles.

Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Merck Millipore Calbiochem™ Protease Inhibitor Cocktail Set III, EDTA-Free

Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Alfa Aesar™ Lysis Buffer, 0.5% Tween 20, pH 8.0

For isolation of His-tag proteins

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns

Designed for method optimization and parameter screening

Thermo Scientific™ Pierce™ Spin Columns - Screw Cap

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads

Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups.

Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

MP Biomedicals™ FastPROTEIN™ BlueMatrix

Easy lysis of bacterial cells to release recombinant proteins

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

Cytiva Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

Thermo Scientific™ PMSF Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules.  Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.

Cytiva Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column

Superdex 30 prep grade (PG) size exclusion chromatography (gel filtration) media prepacked into a HiLoad 26/600 column is optimized for the separation of proteins and peptides, but can also be used with success to separate oligosaccharides.

  spinner