Protein Extraction and Purification

GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors THROMBIN PROTEASE

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

Invitrogen™ Molecular Probes™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays

Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution PROTEIN QUANTITATION KIT ULTRASENSITIVE FLUORESCENce-based protein quantita PROBES KOMBO sufficient

Thermo Scientific™ Low Protein Binding Collection Tubes, 1.5 mL

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL). X2500 Pierce Low Protein Binding MicrocentrifugeTubes, 1.5mL, 10 x 1pk/250 tubes

GE Healthcare Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium. STREPTAVIDIN SEPHAROSE HP,5 ML

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques. X50 Funnel Pierce disposablepolypropylene (pack

GE Healthcare Streptavidin HP MultiTrap™ Plates

Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates X4 STREPTAVIDIN HP MULTITRAP

GE Healthcare C Columns

C columns are economical modular columns for low pressure or gravity feed, providing even distribution of eluent to the medium bed, even without flow adapters. COLUMN GE HEALTHCARE 26/100C FOR LOW PRESSUREgravity feed 26mm x 1000mm

GE Healthcare His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification. X10 HIS GRAVITRAP

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. 10 ML HALT(TM) PROTEASE INHIBITOR COCKTAIL 100X10mL

Thermo Scientific™ Pierce™ Lysozyme

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall. 0.5ML Lysozyme 50mg/mL

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation. 1 ML PIERCE STREPTAVIDIN MAGNETIC BEADS 1ML STOREin Fridge at 4C

Merck™ PureProteome™ Magnetic Stands

Rapidly and easily isolate magnetic particles PureProteome Magnetic Stand, 15mL

GE Healthcare His SpinTrap™ Spin Columns and Kit

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. GE Healthcare Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns. X50 HIS SpinTrap, 100UL

GE Healthcare Mono Q PC, Columns Prepacked with MonoBeads™

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles. MONO Q 4.6/100 PE

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments PROTEASE INH SAMPLE 1 VIAL

Merck™ Calbiochem™ Protease Inhibitor Cocktail Set III, EDTA-Free

Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues. 1ML Protease Inhibitor Cocktail Set III, EDTA-Free Protease Inhibitor Cocktail Set III, EDTA-Free

GE Healthcare Mammalian Protein Extraction Buffer

After preparation may be used for most enzyme assays 500 ML MAMMALIAN PROTEIN EXTRACTION BUFFER 500MLstore at 4°C

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors. HALT PROTEASE&PHOSPHAT INHIB 10ML

Alfa Aesar™ Lysis Buffer, 0.5% Tween 20, pH 8.0

For isolation of His-tag proteins 500ML Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins 500ml

GE Healthcare Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. GE Healthcare Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards. GEL FILTRATION CAL KIT LMW

GE Healthcare HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns

Designed for method optimization and parameter screening 4.7 ML HISCREEN SP FF 4.7ML STORE AT AMBIENTStore at Room Temperature

Thermo Scientific™ Pierce™ Microcentrifuge Spin Cups and Spin Columns

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL). X72 Microcentrifuge tube Pierce Handee 1.5mL

Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads

Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups. NHS-ACTIVATED MAGNETIC BEADS, 5ML

MP Biomedicals™ FastPROTEIN™ BlueMatrix

Easy lysis of bacterial cells to release recombinant proteins 1 SET PROTEIN PURIFICATION KIT 100 PREP (1KIT)

GE Healthcare Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. GE Healthcare Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step. SEPHADEX G-25 COARSE 100 GRS

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories MAGNETIC STAND-96 1 STANDFor the paramagnetic bead precipitation from

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds. 100mL SpeedBead magnetic carboxylate modified particles,DSMG-CM, 1 um, 5% solids

Thermo Scientific™ Pierce™ Protease Inhibitors

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds. E-64, 10 mg

GE Healthcare Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column

Superdex 30 prep grade (PG) size exclusion chromatography (gel filtration) media prepacked into a HiLoad 26/600 column is optimized for the separation of proteins and peptides, but can also be used with success to separate oligosaccharides. HILOAD 26/600 SUPERDEX 30 PG

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