Protein Extraction and Purification

GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors THROMBIN PROTEASE

GE Healthcare Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium. STREPTAVIDIN SEPHAROSE HP,5 ML

Invitrogen™ Molecular Probes™ NanoOrange™ Protein Quantitation Kit, 200-2,000 assays

Sensitive and easy assay for protein quantitation, with detection as low as 10 ng/mL of protein in solution PROTEIN QUANTITATION KIT ULTRASENSITIVE FLUORESCENce-based protein quantita PROBES KOMBO sufficient

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. HALT PROTEASE INHIB 1-USE 2400µL(24x 100µL)

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

Alfa Aesar™ Lysis Buffer, 0.5% Tween 20, pH 8.0

For isolation of His-tag proteins 500ML Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins 500ml

GE Healthcare Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. GE Healthcare Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards. GEL FILTRATION CAL KIT LMW

GE Healthcare C Columns

C columns are economical modular columns for low pressure or gravity feed, providing even distribution of eluent to the medium bed, even without flow adapters. COLUMN C 16/100

Invitrogen™ eBioscience™ RBC Lysis Buffer, 1x

Formulated for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood. eBioscience 1X RBC LysisBuffer 200 ml

GE Healthcare Mammalian Protein Extraction Buffer

500 ML MAMMALIAN PROTEIN EXTRACTION BUFFER 500MLstore at 4°C

GE Healthcare Mono Q PC, Columns Prepacked with MonoBeads™

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles. MONO Q PC 1.6/5

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques. X50 Funnel Pierce disposablepolypropylene (pack

GE Healthcare Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification. 5 ML NI SEPHAROSE(TM) 6 FAST FLOW 5ML STORE ATambient Store at Room Temperature

GE Healthcare HiPrep™ Sephacryl™ S-400 HR Columns

Maximum recovery and minimum nonspecific adsorption 320 ML HIPREP 26/60 SEPHACRYL S-400 HR 320MLstore at ambient Store at Room Temperature

GE Healthcare His SpinTrap™ Spin Columns and Kit

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. GE Healthcare Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns. X50 HIS SpinTrap, 100UL

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Thermo Scientific™ Pierce™ Lysozyme

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall. 0.5ML Lysozyme 50mg/mL

Thermo Scientific™ Pierce™ Protein AG Magnetic Beads

Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation. 5 ML PROTEIN A/G MAGNETIC BEADS, 10MG/ML 5MLStore in Fridge at 4C

GE Healthcare Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. GE Healthcare Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step. SEPHADEX G-25 SUPERFINE 100 GRS

GE Healthcare Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification. Prepacked column, Superdex 200 Increase 10/300 GL

GE Healthcare His Mag Sepharose™ Ni Magnetic Beads

Designed for efficient, small-scale purification/screening of histidine-tagged proteins from different sources GE Healthcare Lifescience™ His Mag Sepharose™ Ni Magnetic Beads enable high-capacity small-scale purification. X2 His Mag Sepharose Ni, 5x 1ml

Thermo Scientific™ Pierce™ Protease Inhibitors

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds. Pepstatin A, 25 mg

GE Healthcare Tricorn™ Superdex™ Prepacked Gel Filtration Columns

Use for laboratory-scale separations and intermediate purification based on different sizes of proteins, peptides, and other biomolecules. GE Healthcare Life Sciences™Tricorn™ Superdex™ Prepacked Gel Filtration Columns are perfect for high-resolution applications. SUPERDEX 75 5/150 GL

Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes. X200 CENTRIFUGE COLUMNS, 0,8 ML

HEPES Lysis Buffer with Triton™ X-100, 2X

125ML HEPES lysis buffer with Triton|r X-100 (2X) 125ml

GE Healthcare illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules.  GE Healthcare illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration. SEPHADEX G-100 DNA GR.100 G

Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay. 240RXN PIERCE ENDOTOXIN QUANT 240RXN 240 RXN

Thermo Scientific™ Low Protein Binding Collection Tubes, 1.5 mL

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL). X2500 Pierce Low Protein Binding MicrocentrifugeTubes, 1.5mL, 10 x 1pk/250 tubes

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Holds up to 24 microcentrifuge tubes MAGNARACK 24each

Invitrogen™ Novex™ SUMO Protease

A recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae SUMO PROTEASE

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