Biological Buffers

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene

Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)

LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C

Invitrogen™ Novex™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MES 20X RUN BUF

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

NUCLEASE-FREE WATER 5 X 100 MLAmbion® Nuclease-free water has not been treated

Invitrogen™ Novex™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled NUPAGE MOPS 20XRUN BUF

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 1 LT 0.5 M EDTA, PH 8.0 1L STORE AT ROOM TEMPERATUre

Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(R)10mL Store at 2-8C

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed. 5 LT RESTORE(TM) WESTERN BLOT STRIPPING BUFFER 5L

Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels NUPAGE TA SDS RNBUF (20X)

Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods. 1 LT BLOCKER CASEIN IN TBS

Lonza™ AccuGENE™ 0.5 M EDTA Solution

AccuGENE Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications. Each reagent is prepared with 18 megOhm water, filtered using a 0.2 micron filter, and filled into sterile bottles. AccuGENE 0.5M EDTA Solution FP=100 ml

Invitrogen™ Novex™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT

Invitrogen™ Phenol:Chloroform:IAA, 25:24:1, pH 6.6

Ready-to-use, safer than preparing crystalline phenol solutions 400 ML PHENOL:CHLOROFORM:IAA, 25:24:1, PH 6.6(raise to pH 7.9 with included buffer) 400mL

MES (Fine White Crystals), Fisher BioReagents

100GR MES monohydrate (Fine White Crystals),

Invitrogen™ MgCl2 (1M)

Ready-to-use, certified RNase-free 1 M MAGNESIUM CHLORIDE 100MLMgCl2solution, 100 ml

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility GLYCO BLUE COPRECIP 5 X 300ULAmbion® GlycoBlue consists of a blue dye

GE Healthcare His Buffer Kit

Purify histidine-tagged proteins under native conditions. His Buffer Kit consists of convenient, premade buffer stock solutions optimized for histidine-tagged protein purification. HIS BUFFER KIT

Invitrogen™ DEPC-Treated Water

DEPC-TREATED WATER 5 X 100 MLAmbion® DEPC-treated water is certified

GE Healthcare Ab Buffer Kit

Premade stock buffers optimized for purification of monoclonal and polyclonal IgG using immobilized Protein A or Protein G 1 SET AB BUFFER KIT 00 STORE IN FRIDGE AT 4C

Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER

Invitrogen™ Novex™ 20X Bolt™ MOPS SDS Running Buffer

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT MOPS 20XRUN BUF

Invitrogen™ TE, pH 8.0

Ready-to-use, certified RNase-free 500 ML TE, PH 8.0 500ML STORE AT ROOM TEMPERATURE

Invitrogen™ Novex™ NativePAGE™ 5% G-250 Sample Additive

Add to samples prepared with the NativePAGE Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE gels NATIVEPAGE 5% G250 ADDITIVENativePAGE 5% G-250 Sample Additive is added to

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Invitrogen™ Novex™ NativePAGE™ Running Buffer Kit

Contains the NativePAGE Running Buffer (20X) NATIVEPAGE RUNNING BUFFER KIT 0 DDNSA # DAYS =155 CDNSA # days = 185 Special Storage Details =

Invitrogen™ Novex™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting 1 LT Transfer buffer NuPAGE(r) (20X), liquid 1L

Invitrogen™ Anza™ 10X Buffer Set

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System. Anza 10X Buffer Set

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC. 1 LT T20 (PBS) BLOCKING BUFFER, SUPERBLOCK 1LStore in fridge at 4C

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