Classical and Basal Cell Culture Media

Classical and Basal Cell Culture Media















Gibco™ Ham's F-12K (Kaighn's) Medium
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Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

Shipping Condition | Room Temperature |
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Content And Storage | Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient |
Form | Liquid |
Without Additives | No HEPES |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Human Hepatocytes |
Sterilization Method | Sterile-filtered |
With Additives | Low Glucose,Glutamine,Phenol Red,Sodium Pyruvate |
Product Type | Ham's F-12K (Kaighn's) Medium |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Gibco™ DMEM, low glucose, GlutaMAX™ Supplement, pyruvate
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DMEM, low glucose, GlutaMAX™ Supplement, pyruvate

Regulatory Status | For in-vitro diagnostic use |
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Content And Storage | Store at 2–8°C. |
Description | DMEM Medium, 500mL, 1 X Concentration, Liquid Form, With Low Glucose, Sodium Pyruvate, GlutaMAX™, Phenol Red, 7 to 7.2 pH, 12 Months Shelf Life, 300 to 340mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture |
With Additives | Low Glucose,Sodium Pyruvate,GlutaMAX,Phenol Red |
Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
Form | Liquid |
Without Additives | No HEPES |
pH | 7 to 7.2 |
Certifications/Compliance | ISO 13485 standards, FDA |
Shelf Life | 12 Months |
Concentration | 1X |
For Use With (Application) | Mammalian cell culture |
Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement
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RPMI 1640 Medium, GlutaMAX™ Supplement
Content And Storage | 2 to 8°C |
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With Additives | GlutaMAX,Phenol Red |
Product Type | Roswell Park Memorial Institute (RPMI) 1640 Medium |
Form | Liquid |
Without Additives | No HEPES,No Sodium Pyruvate |
pH | 7.0 to 7.4 |
Certifications/Compliance | ISO 13485 standards, FDA |
Shelf Life | 12 Months |
Concentration | 1 X |
For Use With (Application) | Mammalian cell culture |
Gibco™ DMEM/F-12, GlutaMAX™ Supplement
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DMEM/F-12, GlutaMAX™ Supplement
Content And Storage | 2 to 8°C |
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Description | DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With GlutaMAX™, Phenol Red, Sodium Pyruvate, 6.8 to 7.2 pH, 12 Months Shelf Life, 290 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture |
With Additives | GlutaMAX,Phenol Red,Sodium Pyruvate |
Product Type | DMEM/F-12 Medium |
Form | Liquid |
Without Additives | No HEPES |
pH | 6.8 to 7.2 |
Certifications/Compliance | ISO 13485 standards, FDA |
Shelf Life | 12 Months |
Concentration | 1 X |
For Use With (Application) | Mammalian cell culture |
Gibco™ DMEM/F-12, HEPES
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DMEM/F-12, HEPES
Regulatory Status | For in-vitro diagnostic use |
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Content And Storage | 2 to 8°C |
Description | DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With L-glutamine, HEPES, Phenol Red, Sodium Pyruvate, 6.9 to 7.3 pH, 12 Months Shelf Life, 270 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture |
With Additives | Glutamine,HEPES,Phenol Red,Sodium Pyruvate |
Product Type | DMEM/F-12 Medium |
Form | Liquid |
pH | 6.9 to 7.3 |
Certifications/Compliance | ISO 13485 standards, FDA |
Shelf Life | 12 Months |
Concentration | 1 X |
For Use With (Application) | Mammalian cell culture |
Gibco™ DMEM/F-12, no glutamine
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Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Form | Liquid |
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Without Additives | No Glutamine,No HEPES |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Shelf Life | 12 Months |
Gibco™ RPMI 1640 Medium, no glutamine
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RPMI 1640 Medium, no glutamine
Regulatory Status | For in-vitro diagnostic use |
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Content And Storage | 2 to 8°C |
Description | RPMI 1640 Medium, 500mL, 1 X Concentration, Liquid Form, With Phenol Red, 7 to 7.4 pH, 12 Months Shelf Life, 260 to 310mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture |
With Additives | Phenol Red |
Product Type | RPMI 1640 Medium |
Form | Liquid |
Without Additives | No Glutamine,No HEPES,No Sodium Pyruvate |
pH | 7 to 7.4 |
Certifications/Compliance | ISO 13485 standards, FDA |
Shelf Life | 12 Months |
Concentration | 1 X |
For Use With (Application) | Mammalian cell culture |
Gibco™ MEM, no glutamine, no phenol red
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MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media.
Gibco™ KnockOut™ DMEM
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KnockOut™ DMEM

Gibco™ Leibovitz's L-15 Medium
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Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.

Shipping Condition | Room Temperature |
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Cell Line | HEP-2 monkey kidney cells |
Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Form | Liquid |
Without Additives | No Glucose,No HEPES,No Sodium Bicarbonate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Sterilization Method | Sterile-filtered |
With Additives | Glutamine,Phenol Red,Sodium Pyruvate,Galactose |
Product Type | Leibovitz's L-15 Medium |
Classification | Animal Origin |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Gibco™ McCoy's 5A (Modified) Medium
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McCoy's 5A (Modified) Medium

Regulatory Status | For in-vitro diagnostic use |
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Content And Storage | 2 to 8°C |
With Additives | High Glucose,Glutamine,Phenol Red,Bacto-peptone |
Product Type | McCoy's 5A (Modified) Medium |
Form | Liquid |
Without Additives | No HEPES,No Sodium Pyruvate |
pH | 6.9 to 7.1 |
Certifications/Compliance | ISO 13485 standards, FDA |
Shelf Life | 12 Months |
Concentration | 1 X |
For Use With (Application) | Mammalian cell culture |
Gibco™ FluoroBrite™ DMEM
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Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standard phenol red–free DMEM.

Content And Storage | Store in refrigerator (2–8°C). Protect from light. |
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With Additives | High Glucose |
Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
Form | Liquid |
Without Additives | No Glutamine,No HEPES,No Phenol Red,No Sodium Pyruvate |
Classification | Animal Origin-free |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Shelf Life | 12 Months From Date of Manufacture |
Concentration | 1 X |
Sterility | Sterile-filtered |
Serum Level | Standard Serum Supplementation |
Gibco™ DMEM, low glucose, pyruvate, no glutamine, no phenol red
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DMEM, low glucose, pyruvate, no glutamine, no phenol red

Regulatory Status | For research use only |
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Content And Storage | 2 to 8°C |
With Additives | Low Glucose,Sodium Pyruvate |
Product Type | DMEM Medium |
Form | Liquid |
Without Additives | No Phenol Red,No Glutamine,No HEPES |
pH | 7 to 7.4 |
Certifications/Compliance | ISO 13485 standards, FDA |
Shelf Life | 12 Months |
Concentration | 1 X |
For Use With (Application) | Mammalian cell culture |
Gibco™ AIM V™ Medium, liquid (research grade)
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AIM V™ Medium, liquid (research grade)

Shipping Condition | Room Temperature |
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Content And Storage | Store in refrigerator (2–8°C) and protect from light. |
Purity or Quality Grade | Research Grade |
Included Antibiotics | Streptomycin Sulfate, Gentamicin |
With Additives | Glutamine,Phenol Red,HSA |
Product Type | Serum Free Medium (SFM) |
Form | Liquid |
Culture Type | Mammalian Cell Culture |
Product Line | AIM V™ |
Cell Type | Monocytes, Dendritic Cells, T Cells, Hybridomas, PBMCs, Fibroblasts, Macrophages, Myeloma Cells, Lymphocytes |