Antibiotics, Buffers and Supplements

Gibco™ Penicillin-Streptomycin (10,000 U/mL)

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria

Gibco™ Trypsin-EDTA (0.25%), phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ ADCF Antifoam Agent, Irradiated

Prevents and minimizes foaming in bioprocess cell culture systems. Cytiva HyClone™ ADCF Antifoam Agent, Irradiated is a gamma radiatopm-sterilized antifoam solution with 3% active simethicone, supplied in a ready-to-use 500mL bag with tube fittings.

Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free

Solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free is tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Gibco™ Trypsin-EDTA (0.05%), phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

Lonza™ Trypsin-Versene™-Trypsin-EDTA Cell Culture Reagent

Combined reagent used to remove cells from culture vessel surface into suspension.Trypsin-Versene™-Trypsin-EDTA Cell Culture Reagent aids in cell-cell adhesion.

Gibco™ GlutaMAX™ Supplement

An alternative to L-glutamine, with increased stability that improves cell health

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ Trypsin (2.5%), no phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red

Unlike porcine trypsin, free of animal-derived components

Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Gibco™ Trypsin-EDTA (0.5%), no phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ Hygromycin B (50 mg/mL)

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ Zeocin™ Selection Reagent

Selection antibiotic, formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus

Lonza™ Mycoalert™ Mycoplasma Detection Kit

Detect mycoplasma in culture. Mycoalert™ Mycoplasma Detection Kit is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.

Gibco™ L-Glutamine (200 mM)

Ready-to-use stock solution that acts as a cell culture supplement

Lonza™ BioWhittaker™ HEPES Buffer

Used in open vessels incubated under atmospheric conditions.  BioWhittaker™ HEPES Buffer is manufactured in accordance with cGMP regulations.

Gibco™ Bacto™ Yeast Extract

Formulation of culture media for cultivation of heterotrophic microorganisms. Gibco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.

Gibco™ Penicillin-Streptomycin (5,000 U/mL)

For prevention from bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria.

Lonza™ Mycoalert™ Mycoplasma Assay Control Set

It is recommended to always include the Control Set to ensure that the operator and all of the assay reagents are performing properly. Mycoalert™ Mycoplasma Assay Control Set is designed for use with the MycoAlert™ Mycoplasma Detection Kit.

Gibco™ B-27™ Supplement (50X), minus vitamin A

Suitable for use in most neuronal cell applications

Gibco™ TrypLE™ Express Enzyme (1X), phenol red

Animal origin-free, recombinant enzyme

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Lonza™ BioWhittaker™ Phosphate Buffered Saline (10X)

Manufactured in accordance with cGMP regulations and cell culture-tested to assure compatibility

Gibco™ N-2 Supplement (100X)

N-2 Supplement (100X)

Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

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