Antibiotics, Buffers and Supplements
Gibco™ Penicillin-Streptomycin (10,000 U/mL)
For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria
Gibco™ Trypsin-EDTA (0.25%), phenol red
Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas
Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free
Solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free is tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Gibco™ Trypsin-EDTA (0.05%), phenol red
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas
Corning™ Matrigel™ Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Cytiva HyClone™ Trypsin Protease
Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.
Gibco™ GlutaMAX™ Supplement
An alternative to L-glutamine, with increased stability that improves cell health
Corning™ Matrigel™ GFR Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Gibco™ Trypsin (2.5%), no phenol red
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas
Gibco™ TrypLE™ Express Enzyme (1X), no phenol red
Unlike porcine trypsin, free of animal-derived components
Gibco™ Sodium Pyruvate (100 mM)
Growth supplement
Gibco™ ACK Lysing Buffer
Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Gibco™ Trypsin-EDTA (0.5%), no phenol red
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas
Gibco™ Hygromycin B (50 mg/mL)
Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus
Gibco™ Trypan Blue Solution, 0.4%
Routinely used as a cell stain to assess cell viability using the dye exclusion test
Gibco™ Zeocin™ Selection Reagent
Selection antibiotic, formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus
Gibco™ L-Glutamine (200 mM)
Ready-to-use stock solution that acts as a cell culture supplement
Gibco™ Bacto™ Yeast Extract
Formulation of culture media for cultivation of heterotrophic microorganisms. Gibco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.
Gibco™ Penicillin-Streptomycin (5,000 U/mL)
For prevention from bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria.
Lonza™ Mycoalert™ Mycoplasma Assay Control Set
It is recommended to always include the Control Set to ensure that the operator and all of the assay reagents are performing properly. Mycoalert™ Mycoplasma Assay Control Set is designed for use with the MycoAlert™ Mycoplasma Detection Kit.
Gibco™ TrypLE™ Express Enzyme (1X), phenol red
Animal origin-free, recombinant enzyme
Corning™ Matrigel™ hESC-Qualified Matrix
Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.
Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
Corning™ Cell-Tak Cell and Tissue Adhesive
Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.
Gibco™ Penicillin-Streptomycin-Glutamine (100X)
For prevention from bacterial contamination in cell cultures due to their effective combined action against gram-positive and gram-negative bacteria.
Gibco™ Antibiotic-Antimycotic (100X)
Anti-mycotic, antibiotic for prevention of cell culture contamination.
Corning™ Cell Recovery Solution
Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses
Lonza™ Trypsin Neutralizing Solution
Used to effectively neutralize Trypsin/EDTA. Lonza™ Trypsin Neutralizing Solution TNS is a component of the ReagentPack™ Subculture Reagent Kit
Kanamycin Sulfate (White Powder), Fisher BioReagents
For selecting transformed cells containing kanamycin resistance gene
Corning™ MEM Nonessential Amino Acid Solution
Suggested replacement for 25-025-CIR