ELISA Reagents

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

Merck Millipore RIPA Lysis Buffer, 10X

For use in immunoprecipitation and Western blotting. RIPA Lysis Buffer, 10X is a concentrated lysis buffer that has been filtered through a 0.2μm membrane and packaged aseptically.

Invitrogen™ Dynabeads™ MyOne™ Streptavidin C1

Exceptional standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets

Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)

Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels

Lonza™ AccuGENE™ 0.5 M EDTA Solution

AccuGENE Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications. Each reagent is prepared with 18 megOhm water, filtered using a 0.2 micron filter, and filled into sterile bottles.

Cytiva (Formerly GE Healthcare Life Sciences) His Buffer Kit

Purify histidine-tagged proteins under native conditions. His Buffer Kit consists of convenient, premade buffer stock solutions optimized for histidine-tagged protein purification.

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Cytiva (Formerly GE Healthcare Life Sciences) Pharmalyte™ Carrier Ampholytes

Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Invitrogen™ eBioscience™ ELISA/ELISPOT Diluent (5X)

Supplemented with fetal bovine serum as source of animal serum proteins. Used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples and making the working dilutions of secondary detection reagent. Also contains 1% ProClin 150 as a preservative

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Cytiva (Formerly GE Healthcare Life Sciences) Ab Buffer Kit

Premade stock buffers optimized for purification of monoclonal and polyclonal IgG using immobilized Protein A or Protein G

Invitrogen™ eBioscience™ IC Fixation Buffer

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)

Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent

For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.

Invitrogen™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Lonza MgCl2 10 mM Solution

Can be used as the sample diluent when attempting to overcome inhibitory chelation effect.

Thermo Scientific™ Pierce™ PNPP Substrate Kit

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer

Thermo Scientific™ Pierce™ TMB Substrate Kit

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ NativePAGE™ Sample Buffer (4X)

A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System

Thermo Scientific™ SuperBlock™ (PBS) Blocking Buffer

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

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