Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Gibco™ DPBS, no calcium, no magnesium
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DPBS, no calcium, no magnesium
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | DPBS |
Gibco™ PBS, pH 7.4
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Shipping Condition | Ambient |
---|---|
Dilution | 1 X |
Content And Storage | 15°C to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.3 to 7.5 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Tris Buffered Saline Tablets, pH 7.6, Medicago
Supplied as exactly pre-weighed tablets. Medicago Tris Buffered Saline Tablets, pH 7.6 gives 500ml of 1x solution with 0.05M Tris-HCl buffer and 0.15M sodium chloride. Manufactured in ISO 9001 and 13485 certified facilities.
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
Gibco™ DPBS (10X), no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 36 months from date of manufacture |
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2630 - 3000 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 6.7 - 7.0 |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
Gibco™ PBS, pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS, pH 7.2
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 280 to 320 mOsm/kg |
Gibco™ PBS (10X), pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS (10X), pH 7.4
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.3 to 7.5 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3100 mOsm/kg |
Gibco™ HBSS (10X), calcium, magnesium, no phenol red
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Tris Hydrochloride, 1M Solution (pH 7.5/Mol. Biol.), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
Synonym | Tris-HCl |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 8.5, 0.2 micron filtered
Invitrogen™ PBS (Phosphate-Buffered Saline) Tablets
Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications.
Thermo Scientific Chemicals MES monohydrate, 99+%, for biochemistry
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 195.23 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic acid monohydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
PubChem CID | 16218417 |
---|---|
CAS | 145224-94-8 |
Molecular Weight (g/mol) | 195.23 |
MDL Number | MFCD00149409 |
SMILES | C1COCCN1CCS(=O)(=O)O.O |
Synonym | 2-(N-Morpholino)ethanesulfonic acid monohydrate |
IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
Molecular Formula | C6H15NO5S |
Gibco™ DPBS (10X), calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DPBS (10X), calcium, magnesium
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
Capacity | 500 mL |
pH | 4.3 to 4.7 |
Shelf Life | 24 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2700 to 3200 mOsm/kg |
Gibco™ DPBS (10X), no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DPBS (10X), no calcium, no magnesium
Dilution | 10 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 6 to 7 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2500 to 2900 mOsm/kg |
Gibco™ DPBS, calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DPBS, calcium, magnesium
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 1 X |
Content And Storage | 2° to 8°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 7 to 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |