Direct PCR Reagents and Kits
Direct PCR Reagents and Kits
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Filtered Search Results
First Strand cDNA Synthesis Kit
Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.
Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit Reaction Mix (5X)
Thermo Scientific™ Maxima™ 5X Reaction Mix is a component of the Maxima First Strand cDNA Synthesis Kits for RT-qPCR (K1671/K1672/K1641/K1642) and may be purchased separately. The 5X Reaction Mix is comprised of reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers.
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Plant |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Shipping Condition | Room Temperature |
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Format | Kit |
Sample Type | PCR Products |
Isolation Technology | Silica Spin Column |
Run Time | 10 min. |
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
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Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Fast or Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, Less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
Shipping Condition | Wet or Dry Ice |
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GC-Rich PCR Performance | Medium |
Includes Label or Dye | Yes |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |
Polymerase | Phire Hot Start II DNA Polymerase |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Tissue |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
Product Type | Multiplex Kit |
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For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
Sample Type | DNA |
Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
Product Line | SNaPshot™ |
Detection Method | Primer-probe |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 2X |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | High-throughput Compatible |
Detection Method | Primer-probe |
Format | Tube |
Product Type | Fast Advanced Cells-to-Ct Kit |
Sample Type | Cell |
Reaction Speed | Fast |
Reagent Type | Cell Lysis |
Purification Target | Lysate |
Product Line | Cells-to-CT™ |
Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
Shipping Condition | Room Temperature |
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Separation Range | 100 bp to 12 kb |
Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
Starting Material Volume | 125 μL |
Isolation Technology | Silica Spin Column |
Elution Volume Max | 20 μL |
For Use With (Application) | Cloning,Standard PCR |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Target | DNA from Enzymatic Reactions (e.g. PCR) |
Format | Spin Column |
Product Type | PCR Micro Kit |
Run Time | 10 min. |
Elution Volume Min | 5 μL |
Product Line | PureLink™ |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Frozen, Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | • Express Lysis Solution (room temperature or 4°C) • Express ezDNase (–20°C) • SuperScript IV VILO Master Mix (–20°C) • SuperScript IV VILO No RT Control (–20°C) • TaqMan Fast Advanced Master Mix (4°C) |
Form | Liquid |
Isolation Technology | Direct cell lysis |
Kit Type | Direct Cell Lysis RT-qPCR |
Green Features | Less hazardous, less waste |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | Yes |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Target | cDNA |
Format | Kit |
Sample Type | Cultured mammalian cells |
Reaction Speed | Fast |
Label or Dye | ROX |
Purification Target | RNA |
Shelf Life | 1 Year |
Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.
Shipping Condition | Dry Ice |
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Sample Type | Cells |
Reaction Speed | Fast or Standard |
Purification Time | 7 min. |
Green Features | Fewer resources used and less waste, less hazardous |
Starting Material | 1–10 Cells |
For Use With (Application) | RT-PCR |
Product Line | Ambion™, Cells-to-CT™ |
Detection Method | Primer-probe |
Invitrogen™ PureLink™ PCR Purification Binding Buffer B2
PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.