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Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Heparin HP Columns
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
Particle Size | 34 μm |
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Ligand Type | 10mg Heparin/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 30°C |
pH Range | 5 to 10 |
Product Type | Heparin HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Sample Type | DNA-binding Proteins, coagulation facto rs, Lipoproteins and Protein synthesis facto rs |
Material | Polypropylene |
For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva HiTrap™ IEX HP Prepacked Columns
Use for high-resolution, small-scale protein purification. Cytiva Life Sciences™ HiTrap™ IEX HP Prepacked Columns are prepacked, ready-to-use Sepharose™ High Performance strong cation exchange columns.
Cytiva HiTrap™ Protein G HP Prepacked Columns
Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.
Content And Storage | 4°C to 30°C |
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Description | HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat. |
Storage Buffer | 20% Ethanol |
Product Type | Pre-packed Columns |
For Use With (Application) | Designed for use with a syringe, peristaltic pump, or chromatography system |
Cytiva HiTrap™ Q Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications. Cytiva Life Sciences™ HiTrap™ Q Sepharose Fast Flow IEX Columns are strong anion exchangers for rapid separations either alone or connected in series.
Content And Storage | 4°C to 30°C in 20% Ethanol |
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Description | HiTrap Q FF |
Column Type | Column Accesory |
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Material | Polypropylene |
Cytiva HiTrap™ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.
Particle Size | 34 μm |
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Ligand Type | 10μM NHS groups/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 8°C |
pH Range | 3 to 12 |
Product Type | NHS-Activated HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Material | Polypropylene |
For Use With (Application) | Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva HiTrap™ SP Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications Cytiva Life Sciences™ HiTrap™ SP Sepharose Fast Flow IEX Columns are strong cation exchanger columns for rapid separations either alone or connected in series.
Content And Storage | 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol |
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Description | HiTrap SP FF |
Cytiva HiTrap™ Phenyl HP Columns
Prepacked columns for convenient and fast separations. Cytiva Lifescience™ HiTrap™ Phenyl HP Columns are designed for use with a syringe, laboratory pump or chromatography system.
Particle Size | 24 to 44 μm |
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Ligand Type | Phenyl |
pH Range | 3 to 13 |
Product Type | HiTrap Phenyl HP |
Material | Polypropylene |
Storage Requirements | 4°C to 30°C, 20% Ethanol |
For Use With (Application) | For Screening of Selectivity, Binding and Elution Conditions, as Well as Small Scale Purifications |
Support Type | 6% Cross-linked Agarose |
Media Type | Phenyl Sepharose HP |
Max. Pressure | 5 bar [0.5 MPa] (70 psi) |
Cytiva HiTrap™ Benzamidine FF (high sub) Columns
Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).
Particle Size | 90 μm |
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Ligand Type | >12μM p-aminobenzamidine/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 2° to 8°C |
pH Range | 2 to 8 |
Product Type | Benzamidine FF Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Sample Type | Serine Proteases such as Thrombin |
Material | Polypropylene |
For Use With (Application) | Removal or purification of Serine Proteases such as Thrombin |
Support Type | 4% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva HiTrap Capto MMC Columns
HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 2 to 12 |
Product Type | HiTrap Capto MMC column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Bind Proteins at the conductivity of the feed material and to solve specific purification challenges |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Capto™ SP ImpRes IEX Columns
High-throughput medium for intermediate and polishing protein purification
Content And Storage | 4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol |
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For Use With (Application) | HiTrap Capto SP ImpRes chromatography column is packed with a strong cation exchange modern resin for high resolution, small-scale protein purification. |
Diameter (Metric) Inner Column | 7 mm |
Particle Size | 90 μm (d50V) |
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Ligand Type | Diethylaminoethyl |
Content And Storage | 4°C to 30°C in 20% Ethanol |
Matrix | Highly cross-linked agarose with dextran surface extender |
pH | 2 to 9 |
Column Material | Polypropylene |
Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
Particle Size | 34μm |
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Buffer | 6M Guanidine Hydrochloride, 8M Urea |
Ligand Type | Iminodiacetic Acid |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 13 |
Product Type | HiTrap chelating HP column |
Column Type | Affinity Column |
For Use With (Application) | IMAC and subsequent purification of Polyhistidine tagged Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 3 bar |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Con A 4B Columns
For separation and purification of glycoproteins, polysaccharides and glycolipids
Particle Size | 45 to 165μm |
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Buffer | Aqueous Buffer |
Ligand Type | Concanavalin A (13mg Con A/mL drained medium) |
Height (Metric) | 25mm |
Content And Storage | 4° to 8°C |
pH Range | 4 to 9 |
Product Type | HiTrap Con A Sepharose 4B column |
Column Type | Ion Exchange Column |
Material | Polypropylene |
For Use With (Application) | Convenient separation and purification of Glycoproteins, Polysaccharides and Glycolipids |
Support Type | 4% Agarose |
Max. Pressure | 3 bar |
Height (English) | 0.98 in. |