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Cytiva HiTrap™ IEX HP Prepacked Columns

Use for high-resolution, small-scale protein purification. Cytiva Life Sciences™ HiTrap™ IEX HP Prepacked Columns are prepacked, ready-to-use Sepharose™ High Performance strong cation exchange columns.

Pricing and Availability

Ion Exchange Columns

Cytiva HiTrap™ Capto™ Adhere Columns

Designed to remove contaminants in post-protein A purification

Pricing and Availability
Specifications

Protein Purification Reagents and Kits

Particle Size	75μm
Buffer	Aqueous Buffer
Ligand Type	N-benzyl-n-methyl Ethanolamine
Height (Metric)	25mm
Content And Storage	4° to 30°C
pH Range	3 to 12
Product Type	HiTrap Capto adhere column
Column Type	Affinity Column
Material	Polypropylene
For Use With (Application)	Easy screening and convenient process development
Support Type	Highly Cross-linked Agarose
Height (English)	0.98 in.

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Pricing and Availability
Specifications

Gel Filtration Media and Columns

Particle Size	34 μm
Ligand Type	10mg Heparin/mL medium
Height (Metric)	2.5cm
Content And Storage	4° to 30°C
pH Range	5 to 10
Product Type	Heparin HP Column
For Use With (Equipment)	Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material	Polypropylene
For Use With (Application)	Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type	6% Highly Cross-linked Agarose
Max. Pressure	42psi
Height (English)	0.98 in.

Cytiva HiTrap™ Q Sepharose Fast Flow IEX Columns

Use for small-scale protein purifications. Cytiva Life Sciences™ HiTrap™ Q Sepharose Fast Flow IEX Columns are strong anion exchangers for rapid separations either alone or connected in series.

Pricing and Availability
Specifications

Ion Exchange Columns

Content And Storage	4°C to 30°C in 20% Ethanol
Description	HiTrap Q FF

Cytiva HiTrap™ SP Sepharose Fast Flow IEX Columns

Use for small-scale protein purifications Cytiva Life Sciences™ HiTrap™ SP Sepharose Fast Flow IEX Columns are strong cation exchanger columns for rapid separations either alone or connected in series.

Pricing and Availability
Specifications

Ion Exchange Columns

Content And Storage	4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol
Description	HiTrap SP FF

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Pricing and Availability
Specifications

Gel Filtration Media and Columns

Particle Size	34 μm
Ligand Type	10μM NHS groups/mL medium
Height (Metric)	2.5cm
Content And Storage	4° to 8°C
pH Range	3 to 12
Product Type	NHS-Activated HP Column
For Use With (Equipment)	Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material	Polypropylene
For Use With (Application)	Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type	6% Highly Cross-linked Agarose
Max. Pressure	42psi
Height (English)	0.98 in.

Cytiva HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

Pricing and Availability
Specifications

Chromatography Media

Particle Size	60 to 160 μm
Ligand Type	Talon Superflow Cobalt-IMAC
Flow Rate	1 to 4 mL/min
Matrix	6% Cross-Linked Agarose
Storage Buffer	20% Ethanol
pH Range	3 to 12
Product Type	HiTrap Talon crude prepacked Column
Material	Polypropylene
Storage Requirements	4°C to 8°C
For Use With (Application)	For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH
Max. Pressure	3 bar

Cytiva HiTrap™ IEX Selection Kit

Use for fast and easy screening of a variety of different media. HiTrap™ IEX Selection Kits contain Sepharose™ Fast Flow and Sepharose XL in combination with seven different ion exchange ligands.

Pricing and Availability

Ion Exchange Columns

Cytiva HiTrap™/HiPrep™ Column Accessories

Pricing and Availability
Specifications

Protein Purification Reagents and Kits

Column Type	Column Accesory
Material	Polypropylene

Cytiva HiTrap Capto MMC Columns

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

Pricing and Availability
Specifications

Protein Purification Reagents and Kits

Particle Size	75μm
Buffer	Aqueous Buffer
Height (Metric)	25mm
Content And Storage	4° to 30°C
pH Range	2 to 12
Product Type	HiTrap Capto MMC column
Column Type	Affinity Column
Material	Polypropylene
For Use With (Application)	Bind Proteins at the conductivity of the feed material and to solve specific purification challenges
Support Type	Highly Cross-linked Agarose
Height (English)	0.98 in.

Cytiva HiTrap™ Protein G HP Prepacked Columns

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

Pricing and Availability
Specifications

Protein Resins

Content And Storage	4°C to 30°C
Description	HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.
Storage Buffer	20% Ethanol
Product Type	Pre-packed Columns
For Use With (Application)	Designed for use with a syringe, peristaltic pump, or chromatography system

Cytiva HiTrap™ IgM Purification HP Column

Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture

Pricing and Availability

Spin Columns and Gravity-flow Columns

Cytiva HiTrap™ Capto™ SP ImpRes IEX Columns

High-throughput medium for intermediate and polishing protein purification

Pricing and Availability
Specifications

Ion Exchange Columns

Content And Storage	4°C to 30°C, 0.2M Sodium Acetate in 20% Ethanol
For Use With (Application)	HiTrap Capto SP ImpRes chromatography column is packed with a strong cation exchange modern resin for high resolution, small-scale protein purification.
Diameter (Metric) Inner Column	7 mm

Cytiva HiTrap Capto S ImpAct Prepacked Columns

HiTrap Capto S ImpAct columns are prepacked with Capto S ImpAct cation exchange chromatography medium for screening and small-scale protein purifications using ion exchange chromatography (IEX).

Pricing and Availability
Specifications

Protein Purification Reagents and Kits

Particle Size	50μm
pH Range	4 to 12
Product Type	HiTrap Capto S ImpAct Prepacked Column
Column Type	Ion Exchange Column
Material	High-flow agarose

Cytiva HiTrap™ Capto™ Q IEX Columns

Strong quaternary ammonium (Q) anion exchanger

Pricing and Availability
Specifications

Ion Exchange Columns

Particle Size	90 μm (d50V)
Ligand Type	Quaternary amine
Content And Storage	4°C to 30°C in 20% Ethanol
Matrix	Highly cross-linked agarose with dextran surface extender
Description	HiTrap Capto Q
pH	2 to 12
Column Material	Polypropylene
For Use With (Application)	HiTrap Capto Q chromatography column is prepacked with a strong anion exchange modern resin for small scale protein purification.

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