Dissociation Reagents

Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ ADCF Antifoam Agent, Irradiated

Prevents and minimizes foaming in bioprocess cell culture systems. Cytiva HyClone™ ADCF Antifoam Agent, Irradiated is a gamma radiatopm-sterilized antifoam solution with 3% active simethicone, supplied in a ready-to-use 500mL bag with tube fittings.

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

Lonza™ Trypsin-Versene™-Trypsin-EDTA Cell Culture Reagent

Combined reagent used to remove cells from culture vessel surface into suspension.Trypsin-Versene™-Trypsin-EDTA Cell Culture Reagent aids in cell-cell adhesion.

Gibco™ GlutaMAX™ Supplement

An alternative to L-glutamine, with increased stability that improves cell health

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ L-Glutamine (200 mM)

Ready-to-use stock solution that acts as a cell culture supplement

Lonza™ BioWhittaker™ HEPES Buffer

Used in open vessels incubated under atmospheric conditions.  BioWhittaker™ HEPES Buffer is manufactured in accordance with cGMP regulations.

Gibco™ B-27™ Supplement (50X), minus vitamin A

Suitable for use in most neuronal cell applications

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Lonza™ BioWhittaker™ Phosphate Buffered Saline (10X)

Manufactured in accordance with cGMP regulations and cell culture-tested to assure compatibility

Gibco™ N-2 Supplement (100X)

N-2 Supplement (100X)

Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Gibco™ CTS™ TrypLE™ Select Enzyme

CTS™ TrypLE™ Select Enzyme

Invitrogen™ Accutase - Enzyme Cell Detachment Medium

Invitrogen™ Accutase Enzyme Cell Detachment Medium performs exceptionally well in detaching cells for analysis of cell surface markers and virus growth assay.

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Invitrogen™ Low Density Lipoprotein from Human Plasma, Acetylated, DiI complex (DiI AcLDL)

Acetylated (Ac) apoprotein used to study cell types that express receptors specific for this acetylated version, i.e., endothelial and microglial cells

Gibco™ KnockOut™ Serum Replacement

KnockOut™ Serum Replacement

Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent

StemPro Accutase Cell Dissociation Reagent

Gibco™ Phytohemagglutinin, M form (PHA-M)

This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1 to 2% v/v

Lonza™ BioWhittaker™ PBS EDTA

Used as a buffer in procedures generating dendritic cells from monocytes.  BioWhittaker™ PBS EDTA is manufactured in accordance with cGMP regulations.

Lonza™ BioWhittaker™ Veronal Buffer (5X)

Manufactured in accordance with cGMP regulations and cell culture-tested to assure compatibility

Lonza™ BioWhittaker™ Sodium Pyruvate Solution (100mM)

Added to cell culture as an additional source of energy since it is a key intermediate in the production of the high energy intermediate ATP within the cell BioWhittaker™ Sodium Pyruvate Solution (100mM) is manufactured in accordance with cGMP regulations.

Lonza™ BioWhittaker™ Hank's Balanced Salt Solutions (1X)

Used in wide variety of tissue culture applications. BioWhittaker™ Hank's Balanced Salt Solutions (1X) is used to maintain a physiological pH for cells maintained in non-CO2 conditions, diluting cells before counting, washing cells before dissociating and transporting cells or tissues.

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