Crosslinking, Labeling and Protein Modification

Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.

Molecular Probes™ Alexa Fluor™ 647 Azide, Triethylammonium Salt

Molecular Probes™ Alexa Fluor™ 647 Azide, Triethylammonium Salt

Alexa Fluor™ 647 azide, triethylammonium salt

Cytiva Cy3 NHS Ester Mono-Reactive CyDye

Cytiva Cy3 NHS Ester Mono-Reactive CyDye

Use for highly accurate labeling of amine groups. Cytiva Cy3 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.

Molecular Probes™ 5-DTAF (5-(4,6-Dichlorotriazinyl) Aminofluorescein), single isomer

Molecular Probes™ 5-DTAF (5-(4,6-Dichlorotriazinyl) Aminofluorescein), single isomer

5-DTAF (5-(4,6-Dichlorotriazinyl) Aminofluorescein), single isomer

Molecular Probes™ 4-Acetamido-4'-Maleimidylstilbene-2,2'-Disulfonic Acid, Disodium Salt

Molecular Probes™ 4-Acetamido-4'-Maleimidylstilbene-2,2'-Disulfonic Acid, Disodium Salt

4-acetamido-4'-maleimidylstilbene-2,2'-disulfonic acid, disodium salt

Molecular Probes™ Alexa Fluor™ 647 Carboxylic Acid, tris(triethylammonium) salt

Molecular Probes™ Alexa Fluor™ 647 Carboxylic Acid, tris(triethylammonium) salt

Alexa Fluor 647 Carboxylic Acid, tris(triethylammonium) salt

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Molecular Probes™ Alexa Fluor™ 546 Carboxylic Acid, tris(triethylammonium) salt

Molecular Probes™ Alexa Fluor™ 546 Carboxylic Acid, tris(triethylammonium) salt

Alexa Fluor 546 Carboxylic Acid, tris(triethylammonium) salt

Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Molecular Probes™ Alexa Fluor™ 647 Alkyne, Triethylammonium Salt

Molecular Probes™ Alexa Fluor™ 647 Alkyne, Triethylammonium Salt

Alexa Fluor™ 647 alkyne, triethylammonium salt

Molecular Probes™ pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)

Molecular Probes™ pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)

pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE)

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Molecular Probes™ 5-TAMRA, SE (5-Carboxytetramethylrhodamine, Succinimidyl Ester), single isomer

Molecular Probes™ 5-TAMRA, SE (5-Carboxytetramethylrhodamine, Succinimidyl Ester), single isomer

5-TAMRA, SE (5-Carboxytetramethylrhodamine, Succinimidyl Ester), single isomer

Molecular Probes™ Fluorescein-5-Isothiocyanate (FITC 'Isomer I')

Molecular Probes™ Fluorescein-5-Isothiocyanate (FITC 'Isomer I')

Fluorescein-5-Isothiocyanate (FITC 'Isomer I')

Merck Millipore Proteinase K

Merck Millipore Proteinase K

From Tritirachium album

Molecular Probes™ Oregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 5-isomer

Molecular Probes™ Oregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 5-isomer

Oregon Green™ 488 Carboxylic Acid, Succinimidyl Ester, 5-isomer

Molecular Probes™ BODIPY™ FL Iodoacetamide (BODIPY™ FL C<sub>1</sub>-IA, <i>N</i>-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-<i>s</i>-Indacene-3-yl)Methyl)Iodoacetamide)

Molecular Probes™ BODIPY™ FL Iodoacetamide (BODIPY™ FL C1-IA, N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-yl)Methyl)Iodoacetamide)

BODIPY™ FL Iodoacetamide (BODIPY™ FL C1-IA, N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-yl)Methyl)Iodoacetamide)

Molecular Probes™ Tetramethylrhodamine-5-Iodoacetamide Dihydroiodide (5-TMRIA), single isomer

Molecular Probes™ Tetramethylrhodamine-5-Iodoacetamide Dihydroiodide (5-TMRIA), single isomer

Tetramethylrhodamine-5-Iodoacetamide Dihydroiodide (5-TMRIA), single isomer

Thermo Scientific™ Pierce™ Dye Removal Columns

Thermo Scientific™ Pierce™ Dye Removal Columns

Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.

Molecular Probes™ BODIPY™ FL (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-<i>s</i>-Indacene-3-Propionic Acid)

Molecular Probes™ BODIPY™ FL (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionic Acid)

BODIPY™ FL (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionic Acid)

Thermo Scientific™ DST (disuccinimidyl tartrate)

Thermo Scientific™ DST (disuccinimidyl tartrate)

Crosslink amines to amines with disuccinimidyl tartrate, composed of NHS-ester groups and a diol-containing spacer arm (6.4A) that is periodate-cleavable.

Molecular Probes™ 5(6)-FAM, SE (5-(and-6)-Carboxyfluorescein, Succinimidyl Ester), mixed isomers

Molecular Probes™ 5(6)-FAM, SE (5-(and-6)-Carboxyfluorescein, Succinimidyl Ester), mixed isomers

5(6)-FAM, SE (5-(and-6)-Carboxyfluorescein, Succinimidyl Ester), mixed isomers

MP Biomedicals™ Pepsin

MP Biomedicals™ Pepsin

From porcine stomach mucosa

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