Protein Modification Reagents

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

MP Biomedicals™ Pepsin

MP Biomedicals™ Pepsin

From porcine stomach mucosa

Thermo Scientific™ Pierce™ Iodoacetamide, Single-Use

Thermo Scientific™ Pierce™ Iodoacetamide, Single-Use

Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping.

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Merck Millipore Novagen™ Overnight Express™ Autoinduction System 1

Merck Millipore Novagen™ Overnight Express™ Autoinduction System 1

Induces protein expression without monitoring cell density.

Thermo Scientific™ Pierce™ Hydroxylamine-HCl

Thermo Scientific™ Pierce™ Hydroxylamine-HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.

Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)

Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)

MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.

Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH

Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Urea

Thermo Scientific™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

Molecular Probes™ DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)

Molecular Probes™ DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)

DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)

Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)

Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Merck Millipore Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

Merck Millipore Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins

Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL)

Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl

Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.

Thermo Scientific™ POROS™ MabCapture™ G Select Resin

Thermo Scientific™ POROS™ MabCapture™ G Select Resin

MabCapture G Select antibody affinity purification resin provides high dynamic binding at low linear flow rates and maintains superior binding capacity at high linear flow rates

Thermo Scientific™ WELQut Protease (5 U/µL)

Thermo Scientific™ WELQut Protease (5 U/µL)

Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags.

Thermo Scientific™ MM(PEG)12 Methyl-PEG-Maleimide Reagent

Thermo Scientific™ MM(PEG)12 Methyl-PEG-Maleimide Reagent

Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units).

Thermo Scientific™ MA(PEG)4 Methyl-PEG-Amine Compound

Thermo Scientific™ MA(PEG)4 Methyl-PEG-Amine Compound

MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications.

Thermo Scientific™ CA(PEG)8 Carboxy-PEG-Amine Compound

Thermo Scientific™ CA(PEG)8 Carboxy-PEG-Amine Compound

CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.

Thermo Scientific™ MM(PEG)24 Methyl-PEG-Maleimide Reagent

Thermo Scientific™ MM(PEG)24 Methyl-PEG-Maleimide Reagent

Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units).

Invitrogen™ Thiol and Sulfide Quantitation Kit

Invitrogen™ Thiol and Sulfide Quantitation Kit

Ultrasensitive colorimetric assay for quantifying both protein and nonprotein thiols

Thermo Scientific™ MA(PEG)8 Methyl-PEG-Amine Compound

Thermo Scientific™ MA(PEG)8 Methyl-PEG-Amine Compound

MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications.

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