Protein Modification Reagents
Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Thermo Scientific™ Pierce™ Iodoacetamide, Single-Use
Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping.
Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Merck Millipore Novagen™ Overnight Express™ Autoinduction System 1
Induces protein expression without monitoring cell density.
Thermo Scientific™ Pierce™ Hydroxylamine-HCl
NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.
Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)
MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.
Thermo Scientific™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ In-Gel Tryptic Digestion Kit
Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
Thermo Scientific™ Immobilized Pepsin (Agarose Resin)
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Merck Millipore Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins
Merck Millipore Calbiochem™ EDTA, Disodium Salt, Dihydrate, Molecular Biology Grade
Application in Molecular Biology
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.
Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl
Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.
Thermo Scientific Pierce™ Pierce™ TCEP-HCl, No-Weigh™ Format
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Thermo Scientific™ CA(PEG)24 Carboxy-PEG-Amine Compound
CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.
Thermo Scientific™ ML(PEG)4 Methyl-PEG-Lipoamide Compound
ML(PEG)n: methyl and bidentate thiol compound with a 4-unit polyethylene glycol (PEG) spacer; methylate and pegylate quantum dots, gold surfaces, silver.
Thermo Scientific™ CL(PEG)12 Carboxy-PEG-Lipoamide Compound
CL(PEG)n: carboxylic acid and bidentate thiol compound with a 4-unit polyethylene glycol (PEG) spacer; carboxylate and pegylate quantum dots, gold surfaces, silver.
Thermo Scientific™ Pierce™ Citraconic Anhydride
Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4).
Thermo Scientific™ Pierce™ Cysteine-HCl
Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.
Thermo Scientific™ Pierce™ Iodoacetic Acid
Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.
Thermo Scientific™ NeuCode™ Lysine-080
Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.
Thermo Scientific™ Pierce™ AEDP (3-[(2-aminoethyl)dithio]propionic acid)
Crosslink amines to carboxyls with this spacer containing an internal disulfide bond; use EDC to modify functional groups on proteins and surfaces.