Cell Culture Media
Lonza™ Endothelial Cell Growth Medium-2 Bullet Kit
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Endothelial Cell Growth Medium-2 Bulletkit is part of a collection of tools optimized to grow certain cell types.
Lonza™ Clonetics™ Bronchial Epithelial Cell Growth Medium Bulletkit
For growth of bronchial/tracheal epithelial cells. Lonza™ Clonetics™ Bronchial Epithelial Cell Growth Medium Bulletkit includes necessary components for primary cell culture.
Gibco™ MEM α, nucleosides, no ascorbic acid
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Gibco™ Leibovitz's L-15 Medium, no phenol red
Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.
Gibco™ Recovery™ Cell Culture Freezing Medium
Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3
Lonza™ Clonetics™ Endothelial Cell Growth Medium BulletKit-2-MV
Used to culture microvascular endothelial cells. Lonza™ Clonetics™ Endothelial Cell Growth Medium BulletKit-2-MV is an optimized media for these primary cells.
Gibco™ Glucose Solution
Gibco™ Glucose Solution contains D-glucose at a concentration of 200 g/L and is used as a serum-free medium supplement suitable for cell culture.
Gibco™ Expi293™ Expression Medium
Expi293™ Expression Medium
Lonza™ SingleQuots™ Endothelial Cell Growth Medium-2MV
Developed for the enhanced growth of lung microvascular cells. Lonza™ SingleQuots™ Endothelial Cell Growth Medium-2MV features a special formulation that is optimized to grow human coronary and iliac artery endothelial cells. Guaranteed to perform when used with Clonetics™ primary cells.
Corning™ DMEM (Dulbecco’s Modified Eagle’s Medium)
Corning™ DMEM (Dulbecco’s Modified Eagle’s Medium) support cell culture growth and substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over original formulation. Products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis.
Gibco™ Collagenase, Type II, powder
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
Gibco™ StemPro™-34 SFM (1X)
A serum-free medium specifically formulated to support the development of human hematopoietic cells in culture.
Gibco™ RPMI 1640 Medium, no glucose
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Gibco™ DMEM/F-12
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Ham's F-12K (Kaighn's) Medium
Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.
Gibco™ MEM
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
Gibco™ DMEM, no glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ B-27™ Supplement (50X), minus vitamin A
Suitable for use in most neuronal cell applications
Gibco™ DMEM/F-12, no phenol red
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Cytiva HyClone™ RPMI 1640 Media
Designed to support cell culture growth and biomanufacturing processes.
Gibco™ DMEM, high glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ MesenPRO RS™ Medium
MesenPRO RS™ Medium
Gibco™ Human Endothelial SFM
Human Endothelial-SFM
Gibco™ N-2 Supplement (100X)
N-2 Supplement (100X)
Gibco™ DMEM/F-12, HEPES
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ Advanced RPMI 1640 Medium
Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Gibco™ IMDM
IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Cell culture media support the growth and maintenance of a variety of mammalian and other cell lines in vitro, as either anchorage-dependent or suspension cultures. Cell culture offers convenience, control, consistency and reproducibility of results, especially when using clonal cells.
Cell culture has multiple applications in cellular and molecular biology:
- Studying normal cell physiology and biochemistry
- Industrial bioprocess/bioreactor manufacturing of vaccines, therapeutic proteins and monoclonal antibodies using recombinant, high-yield protein expression systems
- Pharmaceutical screening and development, including drug metabolism and toxicology
- Other emerging applications in stem cell culture, immunotherapy and cell therapy
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
- Classical mammalian cell culture media (DMEM, MEM, RPMI 1640, McCoy’s, Ham’s, BME and EBSS) contain all the nutrients that cells need, such as amino acids, minerals, vitamins and glucose
- Individual cell culture reagents and supplements, including growth factors (e.g. pyruvate), antibiotics, hormones, cryopreservation media, cell dissociation reagents and animal-derived sera (e.g., FBS foetal bovine serum) which contain a ready-made natural mixture of nutrients
- Speciality media, including stem cell culture media, transfection media, and serum-free or animal-derived component-free (ACDF) additives
- Ancillary buffers and process liquids, including balanced salt solutions (PBS phosphate buffered saline, Hank’s HBSS, Earle’s EBBS), sodium bicarbonate and HEPES buffers, phenol red, cell culture grade water, and adherent cell microcarriers
Cell culture media are typically supplied as presterilised ready-to-use liquids, concentrates, powders or other solids in bottles, or else as single-use sealed bags for integration into compatible cell culture harvesting systems.
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories: